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ez tet plko puro vector addgene  (Addgene inc)


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    Addgene inc ez tet plko puro vector addgene
    Ez Tet Plko Puro Vector Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ez+tet+plko+puro+vector+addgene/EZ-Tet-pLKO-Puro+(Plasmid+%2385966)/pm40209701-531-190-192
    Average 94 stars, based on 45 article reviews
    ez tet plko puro vector addgene - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    CRISPR:

    Article Title: MLKL activity requires a splicing-regulated, druggable intramolecular interaction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NIH-3T3: CRISPR/Cas9 Mlkl-/- Moerke et al.45 N/A HT-29: MlklWT/WT Dr. Klaus Schulze- Osthoff N/A HT-29: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A HT-29: sh_hMLKL0 This paper N/A HT-29: hMLKL0-GFP, hMLKL1-GFP, hMLKL1GFP_hMLKL0-Flag This paper N/A HEK-293T: MlklWT/WT Dr. Frank Essmann N/A A549: MlklWT/WT Dr. Henning Walzack N/A HeLa-hRIPK3 Lafont et al.47 N/A L929: MlklWT/WT Dr. Douglas Green N/A L929: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A Experimental models: Organisms/strains C57BL/6N: Mlklm1/m1, Mlklm2/m2 This paper N/A C57BL/6N: Mlklm1/m1;RipkE-KO, Mlklm2/m2;RipkE-KO This paper N/A C57BL/6N: MlklWT/WT1;RipkE-KO Dannappel et al.28 N/A C57BL/6N: Tnfr-/-;HoipE-KO Peltzer et al.48 N/A Oligonucleotides shRNA targeting hMLKL0: 5’-TGGTAGGGTTCTTTCTGAAAT-3’ This paper N/A crRNA targeting 5’splice donor site in exon 10 of mouse Mlkl: 5’- AGACTCCTACCGTCCACAGA-30 This paper N/A ssODN for generation of Mlklm1/m1: 50-TGAGTTGTTGCGGGAAA TCATTAATGAGTGTCGTGCCCATGAGCCCTCCCAACGGCCCT CgGTGGACGGaAGGAGTCTTTCTGGCAGAGAACGTAAGCCG CGTGTTGATTTCACGATTGTGAAAGGTTTC-30 This paper N/A crRNA targeting 3’splice donor site in exon 10 of mouse Mlkl: 5’-ACAATCGTGAAATCAACACG-3’ This paper N/A ssODN for generation of Mlklm2/m2: 5’-GTGCCCATGAGCCCTC CCAACGGCCCTCTGTGGACGGTAGGAGTCTTTCTGGCAGAG AACcTAAGCtGCGTGTTGATTTCACGATTGTGAAAGGTTTCTT TTGTTTGATTTTTGGTTTCGGCTTTGG-3’ This paper N/A Recombinant DNA Plasmid: Gateway pENTR 11 Dual Selection Vector Invitrogen Cat#A10467 Plasmid: pInducer20 Addgene Cat#44012 Plasmid: pLenti CMV Puro DEST (w118-1) Addgene Cat#17452 Plasmid: PMD2.G Addgene Cat#12259 Plasmid: PSPAX2 Addgene Cat#12260 Plasmid: EZ-Tet-pLKO-Puro vector Addgene Cat#85966 Plasmid: pEGFP-N2 Dr. Frank Essmann N/A Plasmid: pEGFP-N2_mMLKL2_8Ala and This paper N/A Plasmid: pEGFP-N2_mMLKL2_6Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_4Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_2Ala This paper N/A Plasmid: pEGFP-N2_hMLKL1_8aa This paper N/A Plasmid: pEGFP-N2_hMLKL0 This paper N/A Plasmid: pEGFP-N2_hMLKL2_8aa This paper N/A Plasmid: pEGFP-N2_mMLKL2_D448-464 This paper N/A Plasmid: pEGFP-N2_mMLKL2_D457-464 This paper N/A Plasmid: pEGFP-N2_hMLKL1_D462-471 This paper N/A Plasmid: pEGFP-N2_hMLKL2_D254-263 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e12, April 17, 2025

    shRNA:

    Article Title: MLKL activity requires a splicing-regulated, druggable intramolecular interaction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NIH-3T3: CRISPR/Cas9 Mlkl-/- Moerke et al.45 N/A HT-29: MlklWT/WT Dr. Klaus Schulze- Osthoff N/A HT-29: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A HT-29: sh_hMLKL0 This paper N/A HT-29: hMLKL0-GFP, hMLKL1-GFP, hMLKL1GFP_hMLKL0-Flag This paper N/A HEK-293T: MlklWT/WT Dr. Frank Essmann N/A A549: MlklWT/WT Dr. Henning Walzack N/A HeLa-hRIPK3 Lafont et al.47 N/A L929: MlklWT/WT Dr. Douglas Green N/A L929: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A Experimental models: Organisms/strains C57BL/6N: Mlklm1/m1, Mlklm2/m2 This paper N/A C57BL/6N: Mlklm1/m1;RipkE-KO, Mlklm2/m2;RipkE-KO This paper N/A C57BL/6N: MlklWT/WT1;RipkE-KO Dannappel et al.28 N/A C57BL/6N: Tnfr-/-;HoipE-KO Peltzer et al.48 N/A Oligonucleotides shRNA targeting hMLKL0: 5’-TGGTAGGGTTCTTTCTGAAAT-3’ This paper N/A crRNA targeting 5’splice donor site in exon 10 of mouse Mlkl: 5’- AGACTCCTACCGTCCACAGA-30 This paper N/A ssODN for generation of Mlklm1/m1: 50-TGAGTTGTTGCGGGAAA TCATTAATGAGTGTCGTGCCCATGAGCCCTCCCAACGGCCCT CgGTGGACGGaAGGAGTCTTTCTGGCAGAGAACGTAAGCCG CGTGTTGATTTCACGATTGTGAAAGGTTTC-30 This paper N/A crRNA targeting 3’splice donor site in exon 10 of mouse Mlkl: 5’-ACAATCGTGAAATCAACACG-3’ This paper N/A ssODN for generation of Mlklm2/m2: 5’-GTGCCCATGAGCCCTC CCAACGGCCCTCTGTGGACGGTAGGAGTCTTTCTGGCAGAG AACcTAAGCtGCGTGTTGATTTCACGATTGTGAAAGGTTTCTT TTGTTTGATTTTTGGTTTCGGCTTTGG-3’ This paper N/A Recombinant DNA Plasmid: Gateway pENTR 11 Dual Selection Vector Invitrogen Cat#A10467 Plasmid: pInducer20 Addgene Cat#44012 Plasmid: pLenti CMV Puro DEST (w118-1) Addgene Cat#17452 Plasmid: PMD2.G Addgene Cat#12259 Plasmid: PSPAX2 Addgene Cat#12260 Plasmid: EZ-Tet-pLKO-Puro vector Addgene Cat#85966 Plasmid: pEGFP-N2 Dr. Frank Essmann N/A Plasmid: pEGFP-N2_mMLKL2_8Ala and This paper N/A Plasmid: pEGFP-N2_mMLKL2_6Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_4Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_2Ala This paper N/A Plasmid: pEGFP-N2_hMLKL1_8aa This paper N/A Plasmid: pEGFP-N2_hMLKL0 This paper N/A Plasmid: pEGFP-N2_hMLKL2_8aa This paper N/A Plasmid: pEGFP-N2_mMLKL2_D448-464 This paper N/A Plasmid: pEGFP-N2_mMLKL2_D457-464 This paper N/A Plasmid: pEGFP-N2_hMLKL1_D462-471 This paper N/A Plasmid: pEGFP-N2_hMLKL2_D254-263 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e12, April 17, 2025

    Recombinant:

    Article Title: MLKL activity requires a splicing-regulated, druggable intramolecular interaction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NIH-3T3: CRISPR/Cas9 Mlkl-/- Moerke et al.45 N/A HT-29: MlklWT/WT Dr. Klaus Schulze- Osthoff N/A HT-29: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A HT-29: sh_hMLKL0 This paper N/A HT-29: hMLKL0-GFP, hMLKL1-GFP, hMLKL1GFP_hMLKL0-Flag This paper N/A HEK-293T: MlklWT/WT Dr. Frank Essmann N/A A549: MlklWT/WT Dr. Henning Walzack N/A HeLa-hRIPK3 Lafont et al.47 N/A L929: MlklWT/WT Dr. Douglas Green N/A L929: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A Experimental models: Organisms/strains C57BL/6N: Mlklm1/m1, Mlklm2/m2 This paper N/A C57BL/6N: Mlklm1/m1;RipkE-KO, Mlklm2/m2;RipkE-KO This paper N/A C57BL/6N: MlklWT/WT1;RipkE-KO Dannappel et al.28 N/A C57BL/6N: Tnfr-/-;HoipE-KO Peltzer et al.48 N/A Oligonucleotides shRNA targeting hMLKL0: 5’-TGGTAGGGTTCTTTCTGAAAT-3’ This paper N/A crRNA targeting 5’splice donor site in exon 10 of mouse Mlkl: 5’- AGACTCCTACCGTCCACAGA-30 This paper N/A ssODN for generation of Mlklm1/m1: 50-TGAGTTGTTGCGGGAAA TCATTAATGAGTGTCGTGCCCATGAGCCCTCCCAACGGCCCT CgGTGGACGGaAGGAGTCTTTCTGGCAGAGAACGTAAGCCG CGTGTTGATTTCACGATTGTGAAAGGTTTC-30 This paper N/A crRNA targeting 3’splice donor site in exon 10 of mouse Mlkl: 5’-ACAATCGTGAAATCAACACG-3’ This paper N/A ssODN for generation of Mlklm2/m2: 5’-GTGCCCATGAGCCCTC CCAACGGCCCTCTGTGGACGGTAGGAGTCTTTCTGGCAGAG AACcTAAGCtGCGTGTTGATTTCACGATTGTGAAAGGTTTCTT TTGTTTGATTTTTGGTTTCGGCTTTGG-3’ This paper N/A Recombinant DNA Plasmid: Gateway pENTR 11 Dual Selection Vector Invitrogen Cat#A10467 Plasmid: pInducer20 Addgene Cat#44012 Plasmid: pLenti CMV Puro DEST (w118-1) Addgene Cat#17452 Plasmid: PMD2.G Addgene Cat#12259 Plasmid: PSPAX2 Addgene Cat#12260 Plasmid: EZ-Tet-pLKO-Puro vector Addgene Cat#85966 Plasmid: pEGFP-N2 Dr. Frank Essmann N/A Plasmid: pEGFP-N2_mMLKL2_8Ala and This paper N/A Plasmid: pEGFP-N2_mMLKL2_6Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_4Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_2Ala This paper N/A Plasmid: pEGFP-N2_hMLKL1_8aa This paper N/A Plasmid: pEGFP-N2_hMLKL0 This paper N/A Plasmid: pEGFP-N2_hMLKL2_8aa This paper N/A Plasmid: pEGFP-N2_mMLKL2_D448-464 This paper N/A Plasmid: pEGFP-N2_mMLKL2_D457-464 This paper N/A Plasmid: pEGFP-N2_hMLKL1_D462-471 This paper N/A Plasmid: pEGFP-N2_hMLKL2_D254-263 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e12, April 17, 2025

    Plasmid Preparation:

    Article Title: MLKL activity requires a splicing-regulated, druggable intramolecular interaction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NIH-3T3: CRISPR/Cas9 Mlkl-/- Moerke et al.45 N/A HT-29: MlklWT/WT Dr. Klaus Schulze- Osthoff N/A HT-29: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A HT-29: sh_hMLKL0 This paper N/A HT-29: hMLKL0-GFP, hMLKL1-GFP, hMLKL1GFP_hMLKL0-Flag This paper N/A HEK-293T: MlklWT/WT Dr. Frank Essmann N/A A549: MlklWT/WT Dr. Henning Walzack N/A HeLa-hRIPK3 Lafont et al.47 N/A L929: MlklWT/WT Dr. Douglas Green N/A L929: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A Experimental models: Organisms/strains C57BL/6N: Mlklm1/m1, Mlklm2/m2 This paper N/A C57BL/6N: Mlklm1/m1;RipkE-KO, Mlklm2/m2;RipkE-KO This paper N/A C57BL/6N: MlklWT/WT1;RipkE-KO Dannappel et al.28 N/A C57BL/6N: Tnfr-/-;HoipE-KO Peltzer et al.48 N/A Oligonucleotides shRNA targeting hMLKL0: 5’-TGGTAGGGTTCTTTCTGAAAT-3’ This paper N/A crRNA targeting 5’splice donor site in exon 10 of mouse Mlkl: 5’- AGACTCCTACCGTCCACAGA-30 This paper N/A ssODN for generation of Mlklm1/m1: 50-TGAGTTGTTGCGGGAAA TCATTAATGAGTGTCGTGCCCATGAGCCCTCCCAACGGCCCT CgGTGGACGGaAGGAGTCTTTCTGGCAGAGAACGTAAGCCG CGTGTTGATTTCACGATTGTGAAAGGTTTC-30 This paper N/A crRNA targeting 3’splice donor site in exon 10 of mouse Mlkl: 5’-ACAATCGTGAAATCAACACG-3’ This paper N/A ssODN for generation of Mlklm2/m2: 5’-GTGCCCATGAGCCCTC CCAACGGCCCTCTGTGGACGGTAGGAGTCTTTCTGGCAGAG AACcTAAGCtGCGTGTTGATTTCACGATTGTGAAAGGTTTCTT TTGTTTGATTTTTGGTTTCGGCTTTGG-3’ This paper N/A Recombinant DNA Plasmid: Gateway pENTR 11 Dual Selection Vector Invitrogen Cat#A10467 Plasmid: pInducer20 Addgene Cat#44012 Plasmid: pLenti CMV Puro DEST (w118-1) Addgene Cat#17452 Plasmid: PMD2.G Addgene Cat#12259 Plasmid: PSPAX2 Addgene Cat#12260 Plasmid: EZ-Tet-pLKO-Puro vector Addgene Cat#85966 Plasmid: pEGFP-N2 Dr. Frank Essmann N/A Plasmid: pEGFP-N2_mMLKL2_8Ala and This paper N/A Plasmid: pEGFP-N2_mMLKL2_6Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_4Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_2Ala This paper N/A Plasmid: pEGFP-N2_hMLKL1_8aa This paper N/A Plasmid: pEGFP-N2_hMLKL0 This paper N/A Plasmid: pEGFP-N2_hMLKL2_8aa This paper N/A Plasmid: pEGFP-N2_mMLKL2_D448-464 This paper N/A Plasmid: pEGFP-N2_mMLKL2_D457-464 This paper N/A Plasmid: pEGFP-N2_hMLKL1_D462-471 This paper N/A Plasmid: pEGFP-N2_hMLKL2_D254-263 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e12, April 17, 2025

    Selection:

    Article Title: MLKL activity requires a splicing-regulated, druggable intramolecular interaction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NIH-3T3: CRISPR/Cas9 Mlkl-/- Moerke et al.45 N/A HT-29: MlklWT/WT Dr. Klaus Schulze- Osthoff N/A HT-29: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A HT-29: sh_hMLKL0 This paper N/A HT-29: hMLKL0-GFP, hMLKL1-GFP, hMLKL1GFP_hMLKL0-Flag This paper N/A HEK-293T: MlklWT/WT Dr. Frank Essmann N/A A549: MlklWT/WT Dr. Henning Walzack N/A HeLa-hRIPK3 Lafont et al.47 N/A L929: MlklWT/WT Dr. Douglas Green N/A L929: CRISPR/Cas9 Mlkl-/- Gong et al.46 N/A Experimental models: Organisms/strains C57BL/6N: Mlklm1/m1, Mlklm2/m2 This paper N/A C57BL/6N: Mlklm1/m1;RipkE-KO, Mlklm2/m2;RipkE-KO This paper N/A C57BL/6N: MlklWT/WT1;RipkE-KO Dannappel et al.28 N/A C57BL/6N: Tnfr-/-;HoipE-KO Peltzer et al.48 N/A Oligonucleotides shRNA targeting hMLKL0: 5’-TGGTAGGGTTCTTTCTGAAAT-3’ This paper N/A crRNA targeting 5’splice donor site in exon 10 of mouse Mlkl: 5’- AGACTCCTACCGTCCACAGA-30 This paper N/A ssODN for generation of Mlklm1/m1: 50-TGAGTTGTTGCGGGAAA TCATTAATGAGTGTCGTGCCCATGAGCCCTCCCAACGGCCCT CgGTGGACGGaAGGAGTCTTTCTGGCAGAGAACGTAAGCCG CGTGTTGATTTCACGATTGTGAAAGGTTTC-30 This paper N/A crRNA targeting 3’splice donor site in exon 10 of mouse Mlkl: 5’-ACAATCGTGAAATCAACACG-3’ This paper N/A ssODN for generation of Mlklm2/m2: 5’-GTGCCCATGAGCCCTC CCAACGGCCCTCTGTGGACGGTAGGAGTCTTTCTGGCAGAG AACcTAAGCtGCGTGTTGATTTCACGATTGTGAAAGGTTTCTT TTGTTTGATTTTTGGTTTCGGCTTTGG-3’ This paper N/A Recombinant DNA Plasmid: Gateway pENTR 11 Dual Selection Vector Invitrogen Cat#A10467 Plasmid: pInducer20 Addgene Cat#44012 Plasmid: pLenti CMV Puro DEST (w118-1) Addgene Cat#17452 Plasmid: PMD2.G Addgene Cat#12259 Plasmid: PSPAX2 Addgene Cat#12260 Plasmid: EZ-Tet-pLKO-Puro vector Addgene Cat#85966 Plasmid: pEGFP-N2 Dr. Frank Essmann N/A Plasmid: pEGFP-N2_mMLKL2_8Ala and This paper N/A Plasmid: pEGFP-N2_mMLKL2_6Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_4Ala This paper N/A Plasmid: pEGFP-N2_mMLKL2_2Ala This paper N/A Plasmid: pEGFP-N2_hMLKL1_8aa This paper N/A Plasmid: pEGFP-N2_hMLKL0 This paper N/A Plasmid: pEGFP-N2_hMLKL2_8aa This paper N/A Plasmid: pEGFP-N2_mMLKL2_D448-464 This paper N/A Plasmid: pEGFP-N2_mMLKL2_D457-464 This paper N/A Plasmid: pEGFP-N2_hMLKL1_D462-471 This paper N/A Plasmid: pEGFP-N2_hMLKL2_D254-263 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e12, April 17, 2025



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    ez tet plko puro vector addgene  (Addgene inc)
    94
    Addgene inc ez tet plko puro vector addgene
    Ez Tet Plko Puro Vector Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ez+tet+plko+puro+vector+addgene/EZ-Tet-pLKO-Puro+(Plasmid+%2385966)/pm40209701-531-190-192
    Average 94 stars, based on 1 article reviews
    ez tet plko puro vector addgene - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier

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